Yes it will... I used an iPad for navigation and used the same 12v plug
Download navfree USA from the play store and get the Orlando maps.you may need to download a separate voice file too. Worked ok on my iPad.
I'm sure others have told you this, if the hire car company try and sell you an upgraded car, dig your heels in.
They tried to get us to upgrade from a mini van to a massive jeep. Turned out they had run out of mini vans and they had to give us a free upgrade but if we asked for the upgrade then it's money in their pocket. They made us wait thirty mins for the mini van to come from the garage.... But when they realised we weren't gonna get the upgrade the jeep they gave us was ready and waiting. Funny that.
Steve