It would really help if you gave us some more details. What kind of email account? What email app are you using? What sync schedule? What do you use on your pc to retrieve the mail?
Email account is Comcast
Email app on my phone (HTC EVO 3G) is the standard mail app
The sync schedule is set to 4 hours or when I open mail
On my PC is Outlook 2007.
What use to happen was I would be out all day be able to pick-up mail on my phone, reply if needed then when I got home I would logon to my PC start Outlook download all my email, then if I went to my phone and opened my mail account everything would disappear