If you install a custom recovery then OTA updates will not work. Since the normal way of rooting most HTCs is to unlock, flash a custom recovery, then use that to root the ROM, I would guess that you do have one of these.
I did once apply an OTA to a rooted ROM and suffered nothing worse than loosing root, but it can have worse effects than that - it really depends what is in the OTA and what you have changed, so is a bit unpredictable.